Highly efficient method of protein recovery

Existing methods for protein recovery, include enzymatic extraction, single step/conventional isoelectric solubilisation precipitation (ISP), ultrasound (US), pulse electric field (PEF) and microwave assisted extraction. While ISP technology allows rapid recovery of proteins, it results in a low yield as the method is unable to recover all available protein in raw material. To date, ISP alone only retrieves in the range of 40%-80% of total available protein. As a result, most products are of low value. The Solution This novel method involves the use of an improved sequential extraction process based on ISP to utilize this raw waste by-products, allowing recovery of the available protein when processing raw animal material. After the first protein extraction of raw materials, the remaining precipitate is subjected to a second extraction process after pH shifting to extract remaining protein. Applying this method (see Figure) showed recovery of 95% of the total protein available, while increased yields have also been obtained for meat co-products, as well as efficient and economical use of reagents when compared to conventional ISP.