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Confocal Raman microscopy

Confocal Raman microscopy is a powerful technique to provide simultaneous microstructural and chemical information of food materials without the need of staining the samples. This technique is based on the optical principles of confocal microscopy, allowing optical sectioning of the samples and reconstruction of 3D stacks, and the physic-chemical principles of Raman spectroscopy, which allow identifying food ingredients based on their characteristic spectra.

Figure 1. Confocal Raman micrograph of a mixed oil-in-water emulsion containing β-carotene in some of the oil droplets. The image shows the distribution of oil (green), β-carotene (red) and the aqueous phase (blue).