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Dheeraj Rathore

Crop Improvement – Research Technologist

Research Interests

Application of innovative and novel technologies to assist plant breeding, enhance disease resistance and reducing chemical inputs in disease/pest control strategies for sustainable agricultural production.

Current Projects

  • Enhancing the utility of Ensifer-Mediated Transformation (EMT) technology for crop biotechnology. More details: www.emt4crops.com (Teagasc funding)
  • BIOTA – PGPR characterization and analysis (Teagasc funding)
  • Horizontal Gene transfer in microbes (Teagasc funding)
  • Best4Soil: Boosting 4 BEST practices for SOIL health in Europe (EU Horizon2020 funding)
  • CoSTM – Controlling Septoria tritici blotch through crop management (DAFM RSF funding)
  • WheatENHANCE – Breeding of wheat in order to enhance yield and disease resistance by deploying mutation of the BRI1 brassinosteroid receptor gene (DAFM RSF funding)

Education

BSc – Agriculture (2009) Junagadh Agricultural University, India

MSc – Plant Genetics and Crop Improvement (2010) John Innes Centre & University of East Anglia, UK

PhD – Biological & Environmental Science (2017) University College Dublin & Teagasc – Oak Park

Professional Membership

European Plant Science Organisation (EPSO)

International Association for Plant Biotechnology (IAPB)

Rathore DS and Mullins E (2018). Alternative Non‐Agrobacterium Based Methods for Plant Transformation. In Annual Plant Reviews online, J. A. Roberts (Ed.). doi:10.1002/9781119312994.apr0659

Rathore DS, Zuniga-Sotto E, and Mullins E (2018) Ensifer-Mediated Transformation (EMT) of rice (monocot) and oilseed rape (dicot). In Barone P, editor. Transgenic Plants: Methods and Protocols, Methods in Molecular Biology, Springer, Humana Press Inc. New York, https://doi.org/10.1007/978-1-4939-8778-8_3

Rathore DS, Doohan FM and Mullins E (2017). Ensifer-mediated Arabidopsis thaliana Root Transformation (E-ART): A Protocol to Analyse the Factors that Support Ensifer-mediated Transformation (EMT) of Plant Cells. Bio-protocol 7(19): e2564. doi: 10.21769/BioProtoc.2564.

Rathore DS and Mullins E (2017) Ensifer-mediated transformation (EMT): a novel technology platform for the generation of transgenic plants. In Spoel S, editor. The Wheat Issue, GARNish, GARNet for the Arabidopsis and wider Plant Science community, UK, page 30-31.

Rathore DS, Doohan F and Mullins E (2016). “Capability of the plant-associated bacterium, Ensifer adhaerens strain OV14, to genetically transform its original host Brassica napus.” Plant cell, Tissue and Organ Culture: Journal of Plant Biotechnology. doi:10.1007/s11240-016-1032-3

Rathore DS, Lopez-Vernaza MA, Doohan F, Connell DO, Lloyd A and Mullins E (2015). “Profiling antibiotic resistance and electrotransformation potential of Ensifer adhaerens OV14; a non-Agrobacterium species capable of efficient rates of plant transformation.” FEMS Microbiology Letters, 362 (17): fnv126. DOI: http://dx.doi.org/10.1093/femsle/fnv126

Oral Presentations (*All oral presentations were delivered by Rathore DS as first author)

Flash presentation: Enhancing the utility of Ensifer-mediated transformation (EMT) technology. International Association for Plant Biotechnology Congress 2018. (Invited)

Workshop – “Synthetic Biology” at Plant and Animal Genome XXV Conference 2017, San Diego, USA. Presentation title: Enhancing the utility of plant associated bacterium; Ensifer adhaerens Strain OV14, as a vector for plant transformation. (Invited)

Annual Walsh Fellowships Seminar 2015, The Royal Dublin Society, Dublin, Ireland. Presentation title: Developing the commercial and strategic potential of Ensifer-mediated transformation (EMT) – a technology platform for synthesising novel crop varieties.

School of Biology and Environmental Science Post-graduate Seminar 2015, UCD, Dublin, Ireland. Presentation title: Expanding the versatility of Ensifer-mediated transformation (EMT) – a novel technology for the genetic transformation of plant genomes.

The Plant Biology Europe FESPB/EPSO Congress 2014, Dublin, Ireland. Presentation title: Synthesising novel crops using Ensifer-mediated transformation: a technology platform with the potential to contribute positively to global food security.

The Plant Transformation Technology III conference, Vienna, Austria. Presentation title: Expanding the versatility of Ensifer-mediated transformation; a novel method for the genetic transformation of plant genome.

Poster Presentations

Rathore DS, Govindan AK, Zuniga-Sotto E, Doohan F and Mullins E (2019). “Expanding the utility of Ensifer-Mediated Transformation (EMT) technology for crop genetic improvement”, New Phytologist next generation scientists.

Rathore DS, Michel V, Molendjik L, and Gaffney MT (2019). “BEST4SOIL: Developing a decision support tool to guide crop rotation”, Irish Plant Scientists’ Association Meeting.

Rathore DS, Govindan AK, Zuniga-Sotto E, Gelvin SB, Lapham R, David Fitzpatrick D, Doohan F, and Mullins E (2019). “Deciphering the genetic phenomenon that successfully drives the Ensifer-Mediated Transformation (EMT) technology for crop improvement”, Irish Plant Scientists’ Association Meeting.

Rathore DS, Dooley H, Byrne S, Rathore R, Doyle D, Cotter PD, and Steven Kildea (2019). “Studying the impact of fungicides and micronutrients on Septoria tritici blotch of wheat and its associated phylloplane microbiome”, Irish Plant Scientists’ Association Meeting.

Rathore DS, Dooley H, Doyle D and Kildea S (2019). “Controlling Septoria tritici blotch (STB) in wheat through micronutrient management and fungicide applications”, National Tillage Conference.

Rathore DS, Dooley H and Kildea S (2018). “Towards understanding the impact of fungicides and micronutrients on Septoria tritici blotch and wheat phylloplane microbiome”. Society of Irish Plant Pathologists.

Rathore DS, Dooley H and Kildea S (2018). “Investigating the impact of fungicides and micronutrients on Septoria tritici blotch and associated wheat phylloplane microbiome”. Focused Meeting 2018: Microbiomes Underpinning Agriculture.

Rathore DS, Hehir G, Thapa G, Doohan F, Wendt T and Mullins E (2017). “Disease assessment of brassinosteroid (BR)-insensitive 1 (BRI1) mutant lines of barley under Irish agronomic conditions and phenotyping of BRI1 wheat TILLING lines”. PAGXXV conference 2017, San Diego, USA.

Rathore DS, Doohan F, Wendt T and Mullins E (2017). “Enhancing the utility of plant-associated bacterium; Ensifer adhaerens OV14, as a vector for plant transformation”. PAGXXV conference 2017, San Diego, USA. Page 5 of 5

Mullins E, Rudder S, Doohan F, Creevy C, Wendt T, and Rathore DS (2014). “Ensifer-mediated transformation: Elucidating the genetics underpinning this non-Agrobacterium method of T-DNA transfer”. Plant Transformation Technologies III, Vienna, Austria.

Rathore DS, Doohan F, Wendt T and Mullins E (2012). “Developing Ensifer-mediated transformation as a robust platform for genetic manipulation of crop species”. British Society of Plant Pathology Presidential Meeting-2012, Norwich, UK.

Rathore DS, Doohan F and Mullins E (2012). “Expanding our knowledge of Ensifer adhaerens OV14”. Best poster award, Irish Plant Scientist Association Meeting-2012, Dublin, Ireland.